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. 2008 Feb 6;2(2):e147. doi: 10.1371/journal.pntd.0000147

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Njiru et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Sensitivity was compared using preheated and cold template for T. b. rhodesiense isolate LVH 56 (1 and 3) and 058 (2 and 4), respectively, and as monitored using the Rotor-Gene 3000 thermocycler. Preheating of the template accelerates the detection of a positive reaction by approximately 12 min.