Table 2.

Experimental results of growth, glucose uptake, specific glucose uptake, and cAMP concentration in growing cells

Strains	OD600	Glucose uptake	Specific glucose uptakea	cAMP concentration
		M	M/OD	fM
E. coli MG1655 pUC118	0.81±0.17	0.012±0.003	0.015±0.001	10.88±0.28
E. coli MG1655 pUC118-ptsI	0.94±0.34	0.017±0.004	0.018±0.004	7.54±3.71
E. coli MG1655M pUC118	0.67±0.17	0.011±0.003	0.016±0.001	n.t.
E. coli MG1655M pUC118-ptsI	0.11±0.02	0.0029±0.001	0.026±0.008	n.t.

cAMP, cyclic AMP; OD₆₀₀, optical density at 600 nm.

^aThe statistical significance was tested by the Bonferroni t-test. Except for the difference between MG1655 pUC118-ptsI and MG1655 M pUC118, all of the possible combination showed significant difference at least 5% level.

n.t.: not tested.