FIGURE 2. Translocation of γ subunit chimeras and mutants.

A, the γ subunit family is divided as in the cladogram (Fig. 1D) into four groups based on the spatiotemporal properties of translocation. Amino acids mostly conserved within the C-terminal domain of the subunits are highlighted. B, translocation of a wild type and mutant γ₃ subunit. Mutated residues are highlighted in both the wild type and mutant γ₃. Transfected cells were assayed for translocation, and the data are plotted as described in Fig. 1. Shown are representative data (n ≥ 4). C, translocation of chimeric molecules of γ₉ and γ₁₃. Transfected cells were assayed for translocation, and the data are plotted as described in Fig. 1. The images of cells after translocation were overlaid with corresponding images of the same cells expressing Golgi or ER marker. Representative data are shown (n ≥ 5).