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. Author manuscript; available in PMC: 2008 Feb 11.
Published in final edited form as: J Biol Chem. 2007 Jun 20;282(33):24099–24108. doi: 10.1074/jbc.M701191200

FIGURE 4. Effect of different receptors on translocation of γ subunits.

FIGURE 4

Plots of emission intensities from the intracellular membranes (Golgi) as a function of time in CHO cells expressing different receptors as indicated to quantitate potential translocation of YFP-tagged γ subunits as indicated. Transfected cells were assayed for translocation, and the data are plotted as described in Fig. 1. Representative data are shown (n ≥ 4). A, translocation plots in CHO cells transfected with α2 adrenergic receptor (α2AR) tagged with CFP, αo, mCh-β1 and YFP-γ9 subunits. Transfected cells were exposed to 100 μm norepinephrine, and images were captured at the indicated time points followed by exposure to 20 μm yohimbine. B, translocation plots in CHO cells transfected with D2 dopamine receptor tagged with CFP, βo, mCh-β1 and YFP-γ13 subunits. Transfected cells were exposed to 10 μm quinpirole, and images were captured at indicated time points followed by exposure to 100 nm sulpiride. C, translocation plots in CHO cells transfected with α2 adrenergic receptor tagged with CFP, αo, mCh-β1 and YFP-γ10 subunits. Transfected cells were exposed to 100 μm norepinephrine, and images were captured at the indicated time points followed by exposure to 20 μm yohimbine.