Fig. 4.

Salt tolerance test to compare wild type and AtIpk2β expressing tobacco plants. For each experiment, twelve plants of each line were used. (a) Photograph of representative wild type (WT) and transgenic plants (S13, S15) watered with 200-mM NaCl for six weeks. (b), (c) Shoot height and pod numbers of wild type (WT) and transgenic (S13, S15 ) plants treated with 200-mM NaCl. * and ** indicate significant differences in comparison to the wild type at P < 0.05 and P < 0.01, respectively (Student’s t-test). (d) Na⁺ content in leaves and roots of wild type (WT) and transgenic plants (S13, S15) grown in soil supplied with 0 or 200-mM NaCl solution. (e) Photograph of a representative wild type (WT) and transgenic line S15 hydroponically grown in nutrient solution supplemented with 300-mM NaCl for two months. (f) Shoot height and (g) fresh weight of wild type (WT) and transgenic lines S13 and S15 grown for two months under control (0-mM NaCl) or high salt (300-mM NaCl) conditions. Results are presented as means and standard errors from three independent experiments. ** indicates significant differences in comparison to the wild type at P < 0.01 (Student’s t-test)