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. Author manuscript; available in PMC: 2008 Feb 28.
Published in final edited form as: J Biol Chem. 2006 Dec 17;282(8):5570–5581. doi: 10.1074/jbc.M605641200

Figure 3.

Figure 3

Immuno-inhibition by anti-TOGp antibodies of tubulin assembly induced by the DEAE sepharose fraction TOGp mix2 (left graph) or TOGp 200 (right graph). Tubulin assembly is recorded continuously in function of time by measuring the increase in absorbance at 350 nm. Tubulin (12 μM) is polymerized in PEM buffer (solid triangle), an identical experiment is conducted in the presence of 2 μl of anti-TOGp anti serum (empty triangle). Tubulin (12 μM) is polymerized with purified TOGp (30 nM TOGp mix2 or 20 nM TOGp 200) (solid circle), a similar experiment is conducted in the presence of 2 μl of anti-TOGp anti serum (empty circle).