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. Author manuscript; available in PMC: 2008 Feb 28.
Published in final edited form as: J Biol Chem. 2006 Dec 17;282(8):5570–5581. doi: 10.1074/jbc.M605641200

Figure 4.

Figure 4

Estimation of the tubulin critical concentration. Various amounts of tubulin are polymerized at 37°C as indicated in Material and Methods. The curves of assembly of the tubulin control are shown in the inset. The absorbance maximum at 350 nm determined at the end of the polymerization is plotted versus the initial tubulin concentration. The experiment is realised successively with 25 nM TOGp 200, 1 μM taxol and 1 μM colchicine in the presence of 0.5% DMSO (this solvent is required to solubilize taxol and colchicine). The control with tubulin alone is performed both in the presence or in the absence of 0.5% DMSO. Tubulin amount extrapolated through the points to absorbance 0 gives the value of the critical concentration.