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. 1999 Mar 30;96(7):3876–3881. doi: 10.1073/pnas.96.7.3876

Figure 4.

Figure 4

p42-ETS1 up-regulates ICE/caspase-1 expression in DLD1 cells. Expression of Fas apoptotic pathway genes by reverse transcription–PCR analysis. Total RNA was isolated from Jurkat cells, DLD1 parental cells, and various transfectant ETS1-expressing cell lines without treatment with anti-Fas-R antibody CH-11 (A) or from control cells (tTA1) and p42-ETS1-expressing cells (ΔVII-19) after incubation with anti-Fas-R antibody CH-11 (100 ng/ml) at different time points (B). Reverse transcription–PCR analysis was conducted under the following conditions: ICE/caspase-1, 56°C, 40 cycles; CPP32/caspase-3, 54°C, 34 cycles; Fas-R, 56°C, 32 cycles; Fas-L, 60°C, 34 cycles; XIAP, 48°C, 30 cycles; FLIP, 57°C, 38 cycles; and S26, 58°C, 28 cycles. Each of these genes and the size of the products are indicated. These results represent at least four independent experiments.