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. 2008 Feb 20;3(2):e1612. doi: 10.1371/journal.pone.0001612

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Janson et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Plots showing results from one representative donor out of four analyzed. (A) FACS analysis of CD4⁺CD25^lo cells stimulated at day 0 with CD3/CD28 Dynabeads (proportion cell∶beads 1∶1) in presence of 180 U IL-2. Stimuli removed after 48 h and cells followed for an additional 2 weeks with regards to their expression of CD25 (left) and FOXP3 (middle). Left column demonstrating the relationship between CD25 (y-axis) and FOXP3 (x-axis) expression. (B) Expression of CD25 (grey filled bars) and FOXP3 (open bars) in stimulated cells as described above, displayed as mean fluorescent intensity (MFI).