Fig. 2.
Immunization with a plasmid encoding TRP-2 prolongs survival of mice challenged with B16 melanoma cells. Three weeks after vaccination with pcDNA3-trp-2, mice were challenged s.c. in the right flank with 105 B16 melanoma cells. Untreated mice or mice immunized with the empty pcDNA3 vector were used as negative controls. Results represent the sums of two independently performed experiments with 5–10 mice in each treatment group. Immunization with pcDNA3-trp-2 resulted in a significant prolongation of survival (P < 0.0001). Inoculation of the empty pcDNA3 vector also caused a significant prolongation of survival (P = 0.006) as compared with untreated mice.