Table 1.

The five MLST schemes designed for typing bacteria of the B. cereus group

Scheme	Genes	Total sequence length (bp)	Total number of isolatese	Used in (references)
Helgason	adk, ccpA, ftsA, glpT, pyre, recF and sucC	2 938	120	(6,12,46)
Candelon–Sorokina, c	clpC, dinB, gdpD, panC, purF and yhfL	2 850	149	(9,10)
Kob, c	gyrB, mbl, mdh, mutS, pycA(1) and rpoB	2 002	65	(7)
Priesta, b	glpF, gmk, ilvD, pta, purH, pycA(2) and tpi	2 829	721	(8,11,13–15,46–48)
Tourasse–Helgasona, b, d	adk, ccpA, glpF, glpT, panC, pta and pycA(2)	2 658	172	(5)

^aSpecific databases for the Priest and Tourasse–Helgason schemes are accessible at http://pubmlst.org/bcereus/ and http://mlstoslo.uio.no/, respectively. A BLAST database for the Candelon–Sorokin scheme is available at http://spock.jouy.inra.fr/cgi-bin/bacilliMLSopen.cgi.

^bWhile the Tourasse–Helgason and Priest schemes use the same gene fragment for the pycA gene, the Ko scheme is based on a different and non-overlapping gene region.

^cThe B. cereus group-specific transcriptional regulator plcR was originally included in the Candelon–Sorokin and Ko schemes. However, plcR follows a phylogeny different from the other MLST loci (7,10) and is no longer used for MLST; therefore, it is not included in SuperCAT.

^dThe Tourasse–Helgason scheme is a combined scheme based on 3 genes from the Helgason scheme (adk, ccpA, and glpT), 3 genes from the Priest scheme (glpF, pta and pycA(2)), and the panC gene from the Candelon–Sorokin scheme.

^eIncluding strains with fully sequenced genomes.