Figure 2.

Cloning of Th1-specific genes by RDA. (A) Two hundred-microgram PCR products of Driver (Th1) and Tester (Th2), as well as each difference subtraction product DP1, DP2, and DP3, were electrophoresed on 1.5% agarose gel and transferred to Zeta membrane. The membrane was probed by either IFN-γ or GAPDH cDNA probe as indicated. (B) Splenocytes from DO11.10 TCR-transgenic mice were activated with OVA and APCs with either IL-12 or IL-4, as indicated, to initiate Th1 development (lane 1) or Th2 development (lane 2) for 48 hr. Total RNA was prepared and Northern blot analysis was performed by using a full-length ERM cDNA as probe (ERM). The blot was stripped and reprobed for IFN-γ and GAPDH as indicated.