Figure 3.

ERM expression is IL-12-inducible and Stat4-dependent. (A) Wild-type DO11.10 TCR-transgenic splenocytes were activated with OVA and APCs plus various combinations of IL-12, IL-4, and IFN-γ as indicated. On day 7, T cells were harvested and restimulated with OVA and APCs, with (+) or without (−) the addition of IL-12 (10 units/ml). Total RNA was isolated after 48 hr, and Northern blot analysis for ERM, IFN-γ, and GAPDH was performed as above. (B) DO11.10 splenocytes from wild-type, Stat1-deficient, or Stat4-deficient mice DO11.10 mice were activated by OVA in the presence (+) or absence (−) of indicated cytokines for 48 hr. RNA was prepared and Northern blot analysis of ERM, IFN-γ, and GAPDH was performed as described in A. (C) The indicated Ets family members were obtained as expressed sequence tag clones from Genome Systems (St. Louis), and their identity was confirmed by DNA sequencing. Probes were derived from unique coding or 3′ untranslated regions.