Figure 4.

Regulation of ERM expression by IL-12 and IL-18. (A) Th1 cells were generated from DO11.10 TCR-transgenic splenocytes as described above, restimulated with OVA and APCs, and, 7 days later, restimulated with the indicated combinations of anti-CD3, IL-12 (10 units/ml), IL-18 (50 ng/ml), and IFN-γ (100 units/ml) for 48 hr. Total RNA was prepared and Northern blot analysis of ERM, IFN-γ, and GAPDH was performed. (B) Resting 3F6 T cells were stimulated by using anti-CD3-coated plates and IL-12 (10 units/ml) for the indicated times, total RNA was prepared, and ERM and GAPDH Northern blot analysis was performed as described above.