Figure 2.

Cyclization tools incorporated into CyBase. By scanning the distribution of N- to C-termini distances from the PDB as shown in panel (A), the conotoxin MII was identified as a potential target for backbone cyclization. Its relatively short N- to C-termini distance of 9.8 Å means that it is potentially more amenable to backbone cyclization compared to a protein with a longer termini distance. Panel (B) shows a model of a cyclic MII using its native linear structure as a template (PDB ID: 1MII) (37), which has been cyclized in silico using a seven-residue poly-alanine linker (coloured in white). 83 × 136 mm (600 × 600 DPI)