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. Author manuscript; available in PMC: 2008 Feb 12.
Published in final edited form as: J Immunol. 1998 Nov 1;161(9):4563–4571.

FIGURE 3.

FIGURE 3

IL-12 DNA and CD40LT DNA enhance β-gal-specific CTL responses. Mice were vaccinated in a similar manner to that described in Figure 1. Two weeks after the second immunization, total splenocytes were cultured for 5 days at a density of 3 × 106 cells/ml with 1 μg/ml of H-2 Ld-restricted synthetic peptide. Target cells (a β-gal-expressing tumor cell line (CT26.CL25) or peptide-pulsed CT26 wild-type cells) were mixed with effector cells for 6 h at 37°C at E:T ratios, as indicated. A 6-h 51Cr release assay was performed; results are presented as specific lysis. Data shown represent values averaged from four pooled mice with SEM for each E:T ratio. Results are representative of three independent experiments. In all experiments, lysis was <10% using wild-type unpulsed (CT26.WT) targets. Statistical analysis was performed for E:T ratio of 100:1, 50:1, and 25:1. *, p < 0.05 in comparing CTL from mice vaccinated with β-gal plus CD40LT DNA with that from mice vaccinated with β-gal DNA alone.