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. Author manuscript; available in PMC: 2008 Feb 12.
Published in final edited form as: J Immunol. 1998 Nov 15;161(10):5313–5320.

FIGURE 6.

FIGURE 6

Immunization with IL-2-rVV results in an increase in the percentage of Mac-1+ and Gr-1+-double-positive splenocytes. Splenocyte pools used in the experiment shown in Fig. 7 were stained with FITC-anti-Gr-1 and phycoerythrin-anti-Mac-1 mAbs in the presence of an Ab blocking the Fcγ-RII/Fcγ-RIII (2.4G2). Upper panels show unseparated splenocytes from unimmunized mice (naïve) or mice immunized 6 days earlier with either IFN-γ-rVV or IL-2-rVV. Lower panels show the IL-2-rVV-immunized splenocytes after depletion with the indicated mAbs. Not shown are the results of the in vitro depletion with IgG2b mAb used as negative control. As for the in vivo data, depletion with this mAb did not change the percentages of double-positive cells seen in untreated controls. A secondary anti-rat IgG Ab revealed the number of positive cells to be <1% after depletion, confirming that the negativity was not due to Ab competition. Data are representative of four separate, independently performed experiments.