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Localization of Pgp expression with mAb C219 (A) and MRP expression with mAb MRPr1 (B) in cultured neonatal rat CP epithelial cells by confocal microscopy. (Aa and Ba) Top view of the cell layer. (Bar = 10 μm.) (Ab and Bb) Optical sections perpendicular to the plane of the cell layer. Arrowheads in Aa and Ba indicate positions of the respective sections in Ab and Bb. Pgp localizes predominantly toward apical surfaces, whereas MRP localizes toward basolateral surfaces.
