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CSK binds with and phosphorylates c-Jun in vivo. A, c-Jun or CSK was expressed both in the nucleus and cytosol. B, CSK binds with c-Jun in vivo. CSK was co-immunoprecipitated from NIH3T3 cells with a c-Jun antibody. C, CSK binds with c-Jun and phosphorylates c-Jun at tyrosine sites. pACT-csk and pcDNA4-HismaxA-c-jun were transiently transfected into 293T cells, and 40 h later His-tagged proteins were purified followed by Western blot analysis.
