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. Author manuscript; available in PMC: 2008 Dec 1.
Published in final edited form as: DNA Repair (Amst). 2007 Sep 5;6(12):1794–1804. doi: 10.1016/j.dnarep.2007.07.005

Figure 1.

Figure 1

Efficacy of retroviral shRNA directed against p53 in reducing gene expression and protein levels. (A) Effect on basal p53 expression as measured by Taqman in XP-V (GM3617T) and wild-type (GM5659DT) cells. (B) Effect on basal p21 expression as measured by Taqman in XP-V (GM3617T) and wild-type (GM5659DT) cells. (C) Western blots using antibody to ser-15 phospho-p53 showing the basal and damage-inducible p53 protein levels in XP-V (GM3617T) and wild-type (GM5659DT) cells. Bar graphs in the right panels are densitometry plots of band intensities in the adjacent Western blots, with basal p53 levels in vector-treated cells set to 100%. Loading controls, β actin, shown below each western. (D) Relative expression of p21, as a ratio to expression of GUS (see methods) as a function of UV irradiation and shRNA down-regulation of p53.