Figure 1.

Cleavage of peptides spanning the α-secretase cleavage site of APP by ADAM 10. (A) Peptide substrates were incubated in cleavage buffer in the absence (Left) or in the presence of ADAM 10 (Right) for 6 hr at 37°C, followed by HPLC analysis. The asterisks indicate the peptide substrates and the numbers indicate the products generated as follows: 1, EVHHQK-OH; 2, LVFFAEDVGSNK-NH₂; 3, LVFFGEDVGSNK-NH₂. (B) CD spectra of APP peptides. Measurements were carried out in the presence of 0.5% SDS.