Figure 6.

Inhibition effect of a dominant negative ADAM 10 protease form (DN). (A) Endogenous expression of ADAM 10 in HEK and MDBK cells detected by reverse transcriptase–PCR. In each case a 541-bp DNA fragment could be amplified. As control, RNA from HEK cells was treated with RNase A before reverse transcription (St, DNA molecular weight marker). (B) Quantitative analysis of secreted APPsα after immunoblot analysis. The results are expressed as percentage of secreted APPsα in control HEK cells and are the averages ±SD of at least three experiments. Statistical significance between control cells and HEK/DN cells treated or untreated with PMA was determined by Student’s unpaired t test (∗, P < 0.005; ∗∗, P < 0.001).