Table 1.

Specificity of purified ADAM 10 from bovine kidney for peptides spanning the cleavage site of shed proteins

Protein	Sequence	% cleavage by bovine ADAM 10
		30 min	6 hr
APP	EVHHQK ↓ LVFFAEDVGSNK-NH2	28	69
APP (A692G)	EVHHQK ↓ LVFFGEDVGSNK-NH2	14	54
IL-6 receptor	ANATSLPVQ ↓ DSSSV-NH2	0	0
Angiotensin-converting enzyme	TPNSAR ↓ SEGPLPDSGR-NH2	0	0
Pro-TNF-α	PLAQA ↓ VRSSSRTPSD-NH2	49	100

Cleavage of peptides by ADAM 10 after 30 min or 6 hr was determined by HPLC analysis. ↓, proteolytic cleavage site.