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Coimmunoprecipitation of HA-PKD2 and M-TRPC1. HEK293T cells were cotransfected with the indicated combinations of expression plasmids. HA-PKD2 was immunoprecipitated (IP) with α-HA and immunocomplexes were analyzed by immunoblotting (IB) with α-myc (Upper). Expression levels of myc-tagged fusions (M-TRPC1, TRPC3-M, and α1c-M) in the precleared lysates of HEK293T cells before immunoprecipitation are shown (Lower). The positions of molecular mass markers in kDa are shown (Left).
