Colocalization of HA-PKD2 and TRPC1 in live
cells. HEK293T cells were cotransfected with HA-PKD2 and F-TRPC1
(a–c), HA-PKD2 and M-TRPC1
(d–f), or HA-PKD2 and TRPC3-M
(g–i), and the subcellular distribution
of the encoded proteins was determined by double immunofluorescence
using confocal microscopy. FLAG- or myc-tagged proteins were stained
with a FITC-conjugated secondary antibody, while HA-PKD2 was stained
with rhodamine-conjugated secondary antibody. Computerized images of
green fluorescein staining corresponding to F-TRPC1, M-TRPC1, or
TRPC3-M are shown in a, d, and
g, respectively, whereas red rhodamine images
corresponding to HA-PKD2 are shown in b,
e, and h. Fluorescein and rhodamine
merged images corresponding to HA-PKD2 and F-TRPC1, HA-PKD2 and
M-TRPC1, or HA-PKD2 and TRPC3-M subcellular distributions are shown in
c, f, and i,
respectively.