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. 1999 Mar 30;96(7):3934–3939. doi: 10.1073/pnas.96.7.3934

Figure 5.

Figure 5

PKD2-TRPC1 association via a TM region in PKD2. (A) Schematic representation of wild type (1–968; HA-PKD2) or truncation mutants (HA-PKD2/1–379, 1–643, 1–742, 1–871, and 1–702) of HA-PKD2 and summary of results shown in B. Black boxes (■) represent TM segments S1–S6. (B) HEK293T cells were cotransfected with FLAG-tagged bacterial alkaline phosphatase and HA-PKD2 (lane 1), pCDNA3 vector and F-TRPC1 (lane 2), F-TRPC1 and HA-PKD2 (lane 3), HA-PKD2/1–379 (lane 4), HA-PKD2/1–643 (lane 5), HA-PKD2/1–742 (lane 6), HA-PKD2/1–871 (lane 7), or HA-PKD2/1–702 (lane 8). Cells were lysed in IP-500 and HA-tagged proteins were immunoprecipitated (IP) with α-HA. Immunocomplexes were determined by immunoblotting (IB) with α-FLAG (Upper). Expression levels of HA- or FLAG-tagged proteins are shown (Middle or Lower, respectively).