Figure 2.

Effect of RFB4 peptides on the morphology of HUVEC monolayers. HUVEC monolayers were incubated at 37°C for 18 hr with 10⁻⁶ M RFB4-LDV⁺ (A), RFB4-LDV⁻ (B), RFB4-GQT (C), RFB4-LDL⁺ (E), RFB4-LDL⁻ (F), or medium only (D) and then examined by phase-contrast microscopy (magnification ×20). Normal monolayers consisted of highly packed cells with elongated shapes (B–D and F), whereas damaged cells were rounded up and detached from the plate (A and E).