Binding of dgRTA, PE38-lys, and RFB4 peptides to
HUVECs. HUVECs (105) were incubated on ice for 30 min with
FITC reagents in 100 μl PBS/BSA/AZ at varying concentrations,
washed, fixed in 1% paraformaldehyde, and analyzed by flow cytometry.
Values represent the mean ± SD of three experiments.
(A) FITC-dgRTA (▾), FITC-PE38-lys (■),
FITC-carbonic anhydrase (control) (○). Inset shows the
histograms of flow cytometric analyses of the binding of (a) dgRTA, (b)
PE38-lys, and (c) carbonic anhydrase to HUVECs. (B)
FITC-RFB4-LDV+ (▴), FITC-RFB4-LDV−
(▵), FITC-RFB4-GQT+ (◊), FITC-RFB4
(□), FITC-RFB4-LDL+ (●),
FITC-RFB4-LDL− (⧫). Inset shows the
histograms of (a) RFB4-LDV+, (b) RFB4-LDL+, and
(c) RFB4.