Table 3.
Effect of different concentrations of the RFB4 peptide constructs on the morphology of HUVEC monolayers
| Peptides | Concentration, M*
|
|||
|---|---|---|---|---|
| 10-6 | 10-7 | 10-8 | 0 | |
| RFB4-RTA-derived | ||||
| RFB4-LDV+ | ++ | ++ | + | − |
| RFB4-LDV− | − | − | − | − |
| RFB4-GQT+ | − | − | − | − |
| RFB4-IL-2-derived | ||||
| RFB4-LDL+ | ++ | ++ | + | − |
| RFB4-LDL− | − | − | − | − |
| RFB4-dgRTA | ++ | + | + | − |
| RFB4 | − | − | − | − |
HUVECs were grown to confluence in 96-well tissue culture plates and cells were treated for 18 hr with different concentrations of RTA-derived peptide constructs in M199 with 2% fetal calf serum.
*
Morphological changes were scored as: −, no changes; +, rounding up of cells; and ++ disruption and detachment of cells from the cell monolayer.