Figure 1.

(A) RT-PCR analysis of total RNA extracted from pRC/RSV-hUG-transfected and wild-type adenocarcinomas of the uterus (HEC-1A) and the prostate (HTB-81). The PCR products were blotted and detected by hybridization with a hUG-specific oligonucleotide probe, hUGP (21). Amplification of the human GADPH gene was used as an internal control for RNA quality and to rule out pipeting error. Lanes 1 and 2 represent two independently derived pRC/RSV-hUG-transfected clones each of HEC-1A (Left) and HTB-81 (Right), respectively. Wt, wild-type (nontransfected) cells. (B) Western blot analyses showing UG production by HEC-1A and HTB-81 cells transfected with pRC/RSV-hUG. Proteins were resolved by SDS/PAGE under reducing and denaturing conditions (see Materials and Methods for details). UG(d), UG-dimer; UG(m), UG-monomer.