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Anchorage-independent growth on soft agar. (A) HEC-1A cells that were not transfected (wild type). (B) HEC-1A cells that were pRC/RSV (mock)-transfected. (C) HEC-1A cells transfected with pRC/RSV-hUG. Note the striking suppression of anchorage-independent growth of pRC/RSV-hUG-transfected cells (C). Magnification: ×20.
