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Scatchard analysis of specific binding of reduced 125I-UG of wild-type HEC-1A cells (A). Affinity-crosslinking of 125I-hUG with HEC-1A (B) and HTB-81 (C) cells. The cells were incubated with reduced 125I-hUG in the absence or presence of unlabeled reduced hUG for binding and then crosslinked with DSS. Lane 1: (−)DSS; lane 2: (+)DSS, and lane 3: (+)hUG + DSS. Ori, origin. Note the complete absence of the hUG receptor(s) on HTB-81 cells.
