Skip to main content
. 1999 Mar 30;96(7):3975–3980. doi: 10.1073/pnas.96.7.3975

Figure 1.

Figure 1

Generation of TGR(ASrAOGEN). (A) DNA construct used for the production of transgenic rats and the resulting structure of the AS RNA. β-globin intron (striped box), second intron with flanking exonic sequences of the rabbit β-globin gene; poly(A), polyadenylation signal of the bovine growth hormone gene. (B) Identification of transgenic rats by Southern blot analysis. Ten micrograms of genomic DNA of each animal (SD rat) and, as control, 1 ng of plasmid DNA containing the transgene (Co) were digested with PvuII and analyzed by Southern blotting. (C) Detection of AS RNA expression by Northern blot analysis in organs of two lines of TGR(ASrAOGEN) and SD rats. Li, liver; Ki, kidney; He, heart; Ad, adrenal; Br, brain. (D) Localization of AS RNA and AOGEN mRNA in the brain of TGR(ASrAOGEN)1. AOGEN mRNA (Left) and AS RNA (Right) were detected in coronal sections of three different brain areas by in situ hybridization. Sol, nucleus of the solitary tract; 12, hypoglossal nucleus; IO, inferior olive; VL, ventrolateral thalamic nucleus; PAV, paraventricular hypothalamic nucleus; LC, locus coeruleus; B, bregma.