Figure 3.

Immunoblot analysis for PrP in tissues of various mouse lines. (A) Aliquots (120 μg of protein) of tissue homogenates as indicated were loaded per lane. (B) Aliquots (40 μg of protein) of tissue homogenates were digested with 500 units of N-glycosidase F for 2 h at 37°C. (C) Aliquots of tissue homogenates as indicated were immunoprecipitated with 6H4 monoclonal antibody coupled to Sepharose 4B. The eluted proteins were subjected to Western blotting and PrP was detected on blots with 1:10,000 diluted polyclonal anti-PrP antiserum 1B3. Molecular mass markers are indicated on the left in kDa.