Table 1.
Characteristics of transgenic mouse lines
| Line | PrP-encoding gene (copy no.*) | PrP RNA (organ)† | PrP protein (organ)† | Days to symptoms‡ (no. of animals§) |
|---|---|---|---|---|
| Prnp+/+ | Prnp (2) | 194 ± 5 (14/14) | ||
| 1 (br) | 1 (br) | |||
| 1 (spl) | 1 (spl) | |||
| 1 (thy) | 1 (thy) | |||
| Prnp0/0 | – | – | – | >500 (0/10) |
| Tg94/IRF | Eμ/IRF1-PrP (6) | 5 (spl) | >1000 (spl) | 268 ± 24 (18/18) |
| 0.2 (br) | <0.05 (br) | |||
| 3 (thy) | >100 (thy) | |||
| Tg33/lck | lck-PrP (20) | >500 (0/6) | ||
| 2 (spl) | 40 (spl) | |||
| 0.025 (br) | <0.001 (br) | |||
| 40 (thy) | >100 (thy) | |||
| Tg01/alb | alb-PrP (20) | >400 (0/6) | ||
| 11 (liv) | 5 (liv) | |||
| 0.09 (br) | 0.1 (br) | |||
| 0 (spl) | ND | |||
| 0 (thy) | ND |
*
Relative to wild-type; determined by Southern blot analysis. All transgenic animals were homozygous for the transgene and all mice had a mixed 129Sv/C57Bl background.
†
Relative to the corresponding wild-type organ (br, brain; spl, spleen; thy, thymus; liv, liver); determination of PrP mRNA by quantitative Northern blot analysis, and of PrP by densitometry of Western blots. ND, not determined.
‡
Animals were inoculated i.p. with 100 μl of a 1% (wt/vol) brain homogenate (RML isolate).
§
Number of mice developing scrapie/total number of mice inoculated.