Figure 3.

Analysis of the binding specificity of the conformational probes was conducted by phage ELISA as described in Materials and Methods. Estradiol and 4-OH tamoxifen concentrations were 1 μM. The probes α/β I-α/β V are shown only for ER α. The binding patterns of these probes on ER β were similar. Sequences of the probes are as follows: α/β I, SSNHQSSRLIELLSR; α/β II, SAPRATISHYLMGG; α/β III, SSWDMHQFFWEGVSR; α/β IV, SRLPPSVFSMCGSEVCLSR; α/β V, SSPGSREWFKDMLSR; α I, SSEYCFYWDSAHCSR; α II, SSLTSRDFGSWYASR; α III, SRTWESPLGTWEWSR; β I, SREWEDGFGGRWLSR; β II, SSLDLSQFPMTASFLRESR; β III, SSEACVGRWMLCEQLGVSR.