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. 1999 Mar 30;96(7):4005–4010. doi: 10.1073/pnas.96.7.4005

Figure 2.

Figure 2

Detection of envelope SU and p30 CA protein in virions by Western blot. The supernatants from transiently transfected cells were collected and centrifuged through 20% sucrose to pellet viral particles and remove free protein. Pellets were then analyzed on 8–16% SDS/PAGE gels for their gag (p30 CA) and envelope gp70 (SU) content. Viral SU could be detected for all the chimeric envelopes. SU containing the CD33 scFv migrates at a higher position than the wild type. Lanes: 1, mock transfection; 2, ecotropic viral particles from GPE86/LNCX producer cells (41); 3, 33E67; 4, 33E67TR; 5, D84K; 6, 33K67; 7, 33K67TR; 8, CEE+.