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Proton NMR spectrum of fatty acids purified from mutant 155NS1. p-Bromophenacyl esters of fatty acids were collected from the HPLC fractions and were saponified again to remove the p-bromophenacyl group. Free fatty acids were extracted into CDCl3 under acidic conditions and were analyzed with a 500-MHz spectrometer. We show here the spectrum of fatty acids derived from fraction B from HPLC (peak 2 of Fig. 3A) as an example. Assignment of several resonances is shown.
