Table 1.
Adhesion and invasion of PilT+ and PilT− MC onto a monolayer of T84 epithelial cells
Phenotype | Adhesion at 4 h | Adhesion at 9 h
|
Invasion at 9 h* | |
---|---|---|---|---|
Viable cells | Viable cells | Fixed cells | ||
PilT+ | 28.1 ± 1.3 | 13.6 ± 0.5 | 3.1 ± 0.4 | 3.4 ± 0.5 |
PilT− | 33.4 ± 7.2 | 29.0 ± 7.7 | 41.1 ± 0.5 | 3.8 ± 0.6 |
PilE−† | 0.001 ± 0.0 | 0.003 ± 0.001 | ND | ND |
Adhesion is calculated as the ratio of 100 × cell-associated cfu to total cfu. Each value is the mean of three distinct experiments ±SD. ND, not done.
Reported as the log of cell-associated cfu after 60 min of incubation in a medium containing 150 mg/liter of gentamicin. Each value is the mean of at least three different experiments ±SD.
The nonpiliated strain is clone 12pilE∷Km as described (18).