Table 2.
Sequence analysis of transposon-tagged genes reveals homologies with polysaccharide biosynthesis motifs
| Mutant strain | Homologous protein | Degree of homology, % identity/ % similarity | Function of the homologous protein | Organism |
|---|---|---|---|---|
| 38, (2*, 44*, 49*) | Ipi1 | 25.0/33.8 | Isopentenyl-diphosphate delta-isomerase | Arabidopsis thaliana |
| 3 | YwlE | 37.9/51.7 | Protein tyrosine phosphatases | Bacillus subtilis |
| 65, (8, 41) | ExoP | 22.3/35.2 | Polymerization and/or export of succinoglycan | Rhizobium meliloti |
| 21, (24) | EpsD | 74.5/80.1 | NDP-N-acetyl-d-galactosaminuronic acid dehydrogenase | Ralstonia solanacearum |
| 32, (23, 60) | AmsK | 34.1/43.3 | Biosynthesis of amylovoran | Erwinia amylovora |
| 59 (25*) | CysE | 17.0/21.9 | Serine acetyl transferase | Synechocystis PCCC6803 |
| 54, (31, 35, 68) | GumD | 45.0/38.4 | Glycosyl-1-phosphate transferase | Xanthomonas campestris |
| 39 | EpsC | 66.2/69.8 | UDP-N-acetylglucosamine 2-epimerase | R. solanacearum |
| 50 | Cap1K | 16.2/26.5 | UDP-glucose dehydrogenase | Streptococcus pneumoniae |
| 69 | ExoF | 28.9/36.1 | Biosynthesis of succinoglycan | R. meliloti |
| 70 | CapK | 28.9/33.3 | Biosynthesis of type1 capsular polysacchardie | Staphylococcus aureus |
| 74 | ORF11 | 25.8/36.6 | Biosynthesis of serotype K2 capsular polysaccharide | Klebsiella pneumoniae |
| 76 | WcaL | 17.9/28.0 | Glycosyl transferase | Escherichia coli |
| 4, 9, 18, 73 | None |
Based on the identification of homologous sequences of proteins of known function. Degree of homology represented in the third column corresponds to the numbers shown in bold, in the first column.
*
Homologies were determined after identification of overlapping contigs from the V. cholerae O1 tigr sequencing project.