Figure 3.

Characterization of odorant responses in MOR23-adenovirus infected cells. (a) Lyral-mediated Ca²⁺ increase in MOR23 adenovirus-infected cells shown by changes in fura-2 fluorescence intensity ratios (340/380 nm) (1 mM lyral application for 10 sec at the indicated bars). (Upper) Bicistronic MOR23-IRES-GFP. (Lower) Monocistronic GFP. High KCl buffer (HK) and forskolin (FK) (10⁻⁵ M) were applied at indicated bars (5 sec) to assess cell viability and olfactory neuronal properties. (b) Ca²⁺ increase in MOR23-adenovirus infected cells was induced by lyral-containing odorant mixture (mix + LY) but not by the mixture without the lyral (mix − LY). The mixture contains the same odorants used in Fig. 1b (10 or 100 μM). (c) Dose-dependent Ca²⁺ increase as a percentage of the response at 10 mM lyral. Data ±SE (n = 16 for 1 mM, n = 5 for others).