Table 2.
Summary of genes surveyed and primer sequences employed.
| Locus | Functional Annotation via BLAST | Primer Sequences (Forward/Reverse) |
| A19 | At2g21330 | 5'-CTAGAGAACACSGARGCTAACCG |
| Putative fructose bisphosphate aldolase | 5'-TGGCGAAACGRGCACCYTGTTGG | |
| A25 | At2g45740 | 5'-TTGCATGSTCTTATCAGTCC |
| Similar to putative peroxisomal membrane protein PEX11-1 | 5'-GAAGABCCCATCCARCAGAAGAG | |
| A25a | - | Same as A25 Forward |
| 5'-TCTCTCTCATGACACCATGTAAA | ||
| A25b | - | 5'-GCTCCACAGATCAGGCATTT |
| Same as A25 Reverse | ||
| A27 | At3g19900 | 5'-CTTGCAWTGAATGTCATGTGGAAG |
| Unknown protein | 5'-GCTCCCCARCATTTCA | |
| A39 | At2g28315 | 5'-ACTAGTTGGCATYTRATGGTAACA |
| Putative glucose-6-phosphate/phosphate-tranlocator | 5'-GCCRACAAAATTGAGCTGAAGATC | |
| A39a | - | Same as A39 Forward |
| 5'-TCATGGACCAGAAATGAYGTT | ||
| A39b | - | 5'-AGCACCCTTCCYTACTGCAT |
| Same as A39 Reverse | ||
| B7 | At4g27700 | 5'-AGAAAAYARCTTTGTGATTCTTGATG |
| Contains Rhodanese Homology Domain | 5'-GAWGARCAAGCTACTATRATCTTTG | |
| B12 | At3g55800 | 5'-CAAGTGGCTGCAGCCATGGG |
| Sedoheptulose-bisphosphatase precursor | 5'-ACATCRGGMACCATTCCWCCGGTGT | |
| B27 | At4g33250 | 5'-AAGGCTCTTATGGCHATGCC |
| Similar to eukaryotic translation initiation factor 3 subunit 11 | 5'-CGGTTYTTRGCWGCTTCATCCCARAACTG | |
| B27a | - | Same as B27 Forward |
| 5'-TGATGCAAAATAGTTTGTTGGAA | ||
| B27b | - | 5'-TGGTGMTYCTTTTCCAATTC |
| Same as B27 Reverse |
Primer names followed by 'a' or 'b' were designed to amplify a given locus in two parts. Functional annotations were taken from the Genbank record for each Arabidopsis locus. For the unknown protein a BLASTn search failed to yield a putative function