Table 1.

Acyl incorporation into PC and PE, comparing PAM with plasma membranes and an ER-rich fraction

	Acyl incorporation from [14C]18:1-CoA (pmol·min-1·[mg protein]-1)
	PC	PE
Plasma membrane
on its own	53	23
+ lysoPC	549	47
+lysoPC + AgNO3	7	6
PAM-decreased plasma membrane
on its own	89	27
+ lysoPC	329	37
+ lysoPC + AgNO3	11	5
PAM
on its own	74	20
+ lysoPC	840	49
+ lysoPC + AgNO3	14	10
Enriched ER
on its own	91	13
+ lysoPC	899	53
+ lysoPC + AgNO3	15	8

A pea shoot plasma membrane fraction was the starting material for the isolation of the PAM fraction. The PAM-decreased plasma membrane fraction represents the plasma membranes obtained after the removal of the PAM. The ER-enriched fraction is fraction number 1 from the 10-step counter current distribution (see Fig. 1). All fractions were incubated with [¹⁴C]18:1-CoA on its or in the presence of 70 μM lysoPC (acylated with 18:1), without or with 80 μM Ag NO₃. Mean values are presented for 2–5 independent experiments, with standard deviations less than 10%.