Figure 1.

Changes in mRNA expression profiles of MuERVs in distant organs after burn. (A) Schematic representation of primer locations on MuERV. A set of primers (ERV-U2 and ERV-U1) flanking the 3' U3 region are indicated by arrows. (B) RT-PCR analysis of the MuERV expression after burn in the liver, lung, and kidney. Tissues (liver, lung, and kidney) harvested at 3 hours and 24 hours after 18 % TBSA burn were subjected to RT-PCR analysis of MuERV expression using a primer set flanking the 3' U3 region. Respective tissues harvested without any treatment, except for cervical dislocation, serve as a no treatment control in comparison to no burn controls (subjected to anesthesia, resuscitation, and CO₂ inhalation). One representative sample out of three no treatment controls for each tissue is presented. In addition, a genomic MuERV profile was used as a reference.