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(A) Primary murine T cells (CTL) or BMMCs were infected with IRES-GFP-expressing retroviruses additionally expressing murine cystatin F, murine Δ15N cystatin F or GFP only control. GFP-positive cells were sorted by FACS and cathepsin C activity was assayed. BMMC chymase-dependent chloroacetate esterase (CAE) activity was assessed and representative images in (B) were quantitated as shown in (C).
