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Identification of MgtC mutants that are insensitive to downregulation by MgtR. (A) Schematic representation of MgtC protein topology. Mutations that promoted MgtC expression on Western blot are clustered in the second cytoplasmic loop. (B) Western blot analysis of MgtC mutants. MgtC mutations were carried out on the pNM12 plasmid. Extracts were prepared from E. coli strains carrying pMgtR plasmid or the pBBR1MCS vector, and the pNM12 plasmid (mgtC+) or the mutated pNM12 derivatives.
