Figure 1.
The sodium dependence of minocycline transport by human neutrophils. Neutrophils were suspended in HBSS containing NaCl or a Na+-free balanced salts solution containing choline chloride. Minocycline (10 μg/ml) was added and uptake was assayed at 37°C. The data are expressed as the mean (±SEM) transport activity of three experiments. Significant differences in intracellular minocycline content were observed at every time point (P ≤0.01; t test). The insert shows the regression line from Lineweaver-Burk analysis of minocycline transport kinetics (r = 0.98).