Fig. 6.

Iberiotoxin induces a similar depolarization and constriction in cav-1^+/+ and cav-1^−/− arteries, but total arterial K_Ca channel protein is similar. A: membrane depolarization induced by iberiotoxin (100 nM) is similar in pressurized (50 mmHg) cav-1^+/+ (n = 6) and cav-1^−/− (n = 6) arteries. B: iberiotoxin (100 nM) induces a similar constriction in cav-1^+/+ (n = 6) and cav-1^−/− (n = 6) arteries pressurized to 50 mmHg. C: representative Western immunoblots for K_Ca channel α-subunit and β-actin protein in cav-1^+/+ and cav-1^−/− cerebral artery lysates. D: mean data illustrating digital densitometric analysis of bands obtained by Western immuno-blotting for K_Ca channel α-subunit normalized to β-actin (n = 4 separate experiments, with each experiment performed using arteries pooled from 4 mice, giving a total of 16 cav-1^+/+ and 16 cav-1^−/− mice used to obtain data).