Figure 5.

(a) Western blotting analysis of the development (left) and recovery (right) of 30 μM ketoconazole-induced disruption of hERG protein trafficking over time. Data are mean±s.e.m. ^*P<0.05, compared with the control, #P<0.05, compared with just after washing of the drug (recovery 0), 2, 4, 8 and 24 h (n=4–6). (b) Western blotting of cells expressing hERG F656C and Y652A mutant channels under control conditions, and following 48 h incubation with 1–30 μM ketoconazole. Data are mean±s.e.m. ^*P<0.05, compared with the control (n=6).