Eupalmerin acetate (EPA) causes anaesthesia in Xenopus tadpoles. Concentration–response curves for loss of righting reflex (LRR) and loss of swimming reflex (LSR) in tadpoles using EPA as an anaesthetic. The curves were fitted to Hill equation yielding, for LRR, an EC50 of 16.4±0.9 μM and nH of 4.1±0.7, and, for LSR, an EC50 of 20.4±0.01 μM and nH of 58.3±1.4. Each point represents the fraction of 10 animals that met the criteria for LRR or LSR. The points for 1, 3 and 30 μM EPA are the same for LRR and LSR.